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Protein-Protein Interaction Laboratory


 Attila Reményi, PhD

Contact:
Eotvos Lorand University,
Department of Biochemistry
Pázmány Péter sétány 1/C
Budapest
Hungary

Email: remenyi@elte.hu
Tel.: +36 1 2090555/1796 (office) or 8357 (lab)


CV:
 Positions and Employment
1996-1997 Undergraduate Research (Advisor: Chris E. Dempsey) Bristol University, U.K.
1998-2001 Graduate Research (Advisor: Matthias Wilmanns, Hans Schöler) European Molecular Biology (EMBL), Germany
2002-2007 Postdoctoral Fellow (Advisor: Wendell A. Lim) University of California San Francisco (UCSF), Department of Cellular & Molecular Pharmacology, USA
2007-         Associate Research Professor, Eotvos Lorand University, Department of Biochemistry, Hungary

Honors
2006-         Leukemia & Lymphoma Society Special Fellow, USA
2002-2005 Jane Coffin Childs Memorial Fund Postdoctoral Fellowship, USA
1997-2001 EMBL Ph.D. Fellowship, Germany
1996-1997 ELTE-Bristol University Joint Scholarship, UK

Interest:
Proteins are organised into networks. The outcome of many biological processess are determined by protein associations which form in a selective and dnamic manner.  We are intereted in how cellular signalling networks are organized. What molecular interactions determine the architecture of a signaling circuit? What is the molecular basis (the so-called molecular logic) underlying system-level behaviours such as promiscous or selective signal propagation, positive and negative feed-back?
In the lab we use structural biology techniques to address how cellular information is processed.


Expertees:
recombinant DNA techniques, protein expression and purification, X-ray structure determination, protein-protein interaction characterization, modelling

Current lab members:
Attila Reményi, PhD        associate research professor
Anita Alexa, PhD             staff scientist
Tünde Bárkai                   research associate/lab manager
Melinda Lukács               thesis student
Ágnes Garai                     thesis student

Projects:

3D organization of yeast MAP-kinase modules

Specificity in kinase and protease mediated protein-protein interactions

Protein scaffolds in MAP-kinase mediated signal transduction


Publications:

Reményi, A., Good, M.C., Lim, W.A. (2006) Docking interactions in protein kinase and phosphatase networks.

            Current Opinion in Structural Biology 16, 676-685

Bhattacharyya, R.P., Reményi, A., Yeh, B.J., Lim, W.A. (2006) Domains, Motifs, and Scaffolds: the role of modular interactions in the evolution and wiring of cell signalling circuits.

Annual Review of Biochemistry 75, 655-680


Bhattacharyya, R.P.*, Reményi, A.*, Good, M.C., Bashor, C, Falick, A, Lim, W.A. (2006) Ste5 allosterically modulates signaling output of the yeast mating pathway.

Science 311, 822-826 *Equal contribution


Reményi, A., Good, M.C., Bhattacharyya, R.P., Lim, W.A. (2005) The role of docking interactions in mediating signalling input, output, and discrimination in the yeast MAP kinase network.

            Molecular Cell 20, 951-952


Reményi, A., R., Schöler, H.R., Wilmanns, M. (2004) Combinatorial control of gene expression.

Nature Strucure & Molecular Biology 11, 812-815


Reményi, A., Lins, K., Nissen L.J., Reinbold, R., Schöler, H.R., Wilmanns, M. (2003) Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.

Genes & Development 17, 2048-2059


Lins, K., Reményi, A., Tomilin, A., Massa S., Wilmanns, M, Matthias, P., Schöler, H.R. (2003) OBF1 enhances transcriptional potential of Oct1.

        EMBO Journal 22, 2188-2198


Reményi, A., Tomilin, A., Schöler, H.R., Wilmanns, M. (2002) Differential activity by DNA-induced quarternary structures of POU transcription factors.

Biochemical Pharmacology 64, 979-984


Reményi, A., Tomilin, A., Pohl, E., Lins, K., Philippsen, A., Reinbold, R., Schöler, H.R., Wilmanns, M. (2001) Differential dimer activities of the transcription factor Oct-1 by DNA-induced interface swapping.

Molecular Cell 8, 569-580


Reményi, A., Pohl, E., Schöler, H.R, Wilmanns, M. (2001) Crystallization of redox-insensitive Oct-1 POU domain with different DNA-response elements.

Acta Crystallographica D 57, 1634-1638


Tomilin*, A., Reményi*, A., Lins, K., Bak, H., Leidel, S., Vriend, G., Wilmanns, M., and Schöler, H. R. (2000) Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration.

Cell 103, 853-864 *Equal contribution



Takei, J., Reményi, A., Dempsey, C.E. (1999) Generalised bilayer perturbation from peptide helix dimerisation at membrane surfaces: vesicle lysis induced by disulphide-dimerised melittin analogues.

FEBS Letters 442, 11-14


Takei, J., Reményi, A., Clarke, A.R and Dempsey, C.E (1998) Self-association of disulfide-dimerized melittin analogues.

Biochemistry 37, 5699-5708